Determination of Total Urinary Protein by Combined Gel Filtration and Automated Biuret Reaction
نویسندگان
چکیده
منابع مشابه
Determination of total urinary protein by combined gel filtration and automated biuret reaction.
Determination of total urinary protein is now more frequently requested of the clinical chemistry laboratory, one reason being the increased number of patients with renal transplants for whom total urinary protein is determined as an index of immunological rejection (1). Available manual methods are cumbersome and inefficient under clinical laboratory conditions. Concentrations for total urinar...
متن کاملCombined gel filtration, biuret/copper method compared with an immunochemical method for urinary protein measurement.
We compared an immunochemical method specific for plasma proteins with a chemical method, in which interfering substances are separated by gel filtration and "total" urinary protein is determined by the biuret reaction followed by reaction of protein-bound copper with diethyldithiocarbamate after a second gel filtration to remove nonprotein-bound copper. More than 250 24-h urine samples were an...
متن کاملGel filtration. Biuret method for determination of total protein in cerebrospinal fluid.
Interfering low molecular weight substances were removed from cerebro spinal fluid by gel filtration using Sephadex G-50-80 and total protein concen tration of the effluent was determined by the biuret reaction. Results of the proposed method were higher than comparable results obtained by a trichloro acetic acid turbidimetric method. Reproducibility of the method is acceptable.
متن کاملDetermination of serum proteins by means of the biuret reaction.
In the course of an investigation of the biochemical changes following experimental liver injury we felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum. Among the simpler procedures known, the biuret reaction seemed to offer the most encouraging possibilities. Variations and improvements in the application of the biuret reaction ...
متن کاملQuantitation of sputum protein by use of the biuret reaction.
A simple, accurate method is described for quantitatively determining the protein content of sputum by use of the biuret reaction. Fitteen mechanically homogenized specimens were examined with the biuret reagent described by Rosenthal and Cundiff, and the results compared with those obtained by the microKjeldahl technique. Over a range of 542 to 1917 mg of protein per 100 ml, the techniques cor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1972
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/18.3.296